Freeze-Driven Synthesis of DNA Hairpin-Conjugated Gold Nanoparticle Biosensors for Dual-Mode Detection. Academic Article uri icon


  • Freeze-based immobilization of deoxyribonucleic acid (DNA) oligonucleotides on gold nanoparticles (AuNPs) is highly efficient for single-stranded oligonucleotides but typically does not accommodate structures such as snap-cooled DNA hairpins (Sc-HPs) and snap-cooled molecular beacons (Sc-MBs) frequently used for biorecognition applications. Recognizing this limitation, we have developed a modified, freeze-based technique specifically designed to enable the adsorption of such hairpin oligonucleotides onto AuNP surfaces while ensuring that they retain their biosensing capabilities. Successful hairpin oligonucleotide conjugation of varying lengths to a wide range of AuNP diameters was corroborated by dynamic light scattering, -potential, and UV-vis spectrophotometry. Moreover, we conducted a thorough evaluation of this modified method, confirming the retention of the sensing functions of Sc-HPs and Sc-MBs. This advancement not only offers a more efficient route for DNA hairpin conjugation but also elucidates the underlying biorecognition functions, with implications for broader applications in molecular diagnostics.

published proceedings

  • ACS Appl Bio Mater

author list (cited authors)

  • San Juan, A. M., Jaitpal, S., Ng, K. W., Martinez, C., Tripathy, S., Phillips, C., Cot, G. L., & Mabbott, S.

complete list of authors

  • San Juan, Angela Michelle||Jaitpal, Siddhant||Ng, Ka Wai||Martinez, Cecilia||Tripathy, Sayantan||Phillips, Christian||Coté, Gerard L||Mabbott, Samuel

publication date

  • April 2024