FAM20A is a golgi-localized Type II transmembrane protein. Academic Article

abstract

• Family with sequence similarity 20, member A (FAM20A) is a pseudo-kinase in the secretory pathway and is essential for enamel formation in humans. Here we examine if FAM20A is a membrane-associated protein. We show that the full-length FAM20A can be purified from HEK293 cells transfected with a FAM20A-expresing construct. Further, it is only found in the membrane fraction, but not in the soluble fraction, of cell lysate. Consistently, it is not secreted out of the expressing cells. Moreover, it is co-localized with GM130, a cis-Golgi network marker, and membrane topology analysis indicates that it has its C-terminus oriented towards the lumen of the organelle. Our results support that FAM20A is a Type II transmembrane protein within the secretory compartments.

authors

• Lu, Yongbo

published proceedings

• Sci Rep

author list (cited authors)

• Siddiqui, M. F., Li, J., Wang, S., Zhang, H., Qin, C., & Lu, Y.

complete list of authors

• Siddiqui, Mohammad Faizan||Li, Jiahe||Wang, Suzhen||Zhang, Hua||Qin, Chunlin||Lu, Yongbo

publication date

• March 2024

publisher

• Springer Nature  Publisher

published in

• Scientific Reports  Journal

keywords

• Dental Enamel Proteins
• Golgi Apparatus
• HEK293 Cells
• Humans
• Membrane Proteins
• Phosphotransferases

PubMed Central ID

• 38499693

Digital Object Identifier (DOI)

• 10.1038/s41598-024-57007-z

start page

• 6518

volume

• 14

issue

• 1

URL

• http://dx.doi.org/10.1038/s41598-024-57007-z