A protocol for whole-brain Ca2+ imaging in Astyanax mexicanus, a model of comparative evolution.

abstract

• In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca2+ sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb.

authors

• Keene, Alex

published proceedings

• STAR Protoc

author list (cited authors)

• Lloyd, E., Privat, M., Sumbre, G., Dubou, E. R., & Keene, A. C.

citation count

• 0

complete list of authors

• Lloyd, Evan||Privat, Martin||Sumbre, German||Duboué, Erik R||Keene, Alex C

publication date

• September 2023

publisher

• Elsevier  Publisher

published in

• STAR Protocols  Journal

keywords

• Evolutionary Biology
• Neuroscience

PubMed Central ID

• 37742184

Digital Object Identifier (DOI)

• 10.1016/j.xpro.2023.102517

start page

• 102517

end page

• 102517

volume

• 4

issue

• 4

URL

• http://dx.doi.org/10.1016/j.xpro.2023.102517