UTILIZATION OF CULTURED CHICK-EMBRYO HEPATOCYTES AS INVITRO BIOASSAYS FOR POLYCHLORINATED-BIPHENYLS (PCBS) - QUANTITATIVE STRUCTURE-INDUCTION RELATIONSHIPS
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abstract
The concentration-dependent induction of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin-O-deethylase (EROD) activities by several PCB congeners was determined in primary chick embryo hepatocytes in culture. The most active inducers were the coplanar PCBs, 3,3,4,4-tetra- and 3,3,4,45-pentachlorobiphenyl (pentaCB) with EC50 values of 1.2 10-9 and 1.5 10-9 M (AHH induction) and 2.3 10-9 and 1.2 10-9 M (EROD induction) respectively. The corresponding EC50 values for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were 2.4 10-11 and 7.2 10-11 M for AHH and EROD induction respectively. Three monoortho coplanar PCB analogs, 2,3,3,4,4-pentaCB, 2,3,4,4,5-pentaCB and 2,3,3,4,4,5-hexaCB also exhibited induction activity but their corresponding EC50 values were much higher than observed for the coplanar PCBs. Several di- and triortho-substituted PCB congeners were weakly active as inducers of AHH and EROD activity. The structure-induction relationships for the coplanar and monoortho coplanar PCBs in the chick embryo hepatocytes were comparable to those previously reported in rat hepatoma H-4-II E cells and in in vivo studies. 1990.
