Identification of variants of the SPINK1 gene and their association with pancreatitis in Miniature Schnauzers. Academic Article uri icon

abstract

  • OBJECTIVE: To evaluate the serine protease inhibitor, Kazal type 1 (SPINK1) gene for variants and to determine their possible association with pancreatitis in Miniature Schnauzers. ANIMALS: 39 Miniature Schnauzers with pancreatitis, 25 healthy Miniature Schnauzers, and 23 healthy dogs of other breeds. PROCEDURES: The entire canine SPINK1 gene with its intron-exon boundaries was initially sequenced in 22 Miniature Schnauzers. Then, 2 regions of the gene were sequenced in 65 additional canine DNA samples at the locations of variants identified in the initial sequencing of the entire SPINK1 gene. RESULTS: Analysis of the SPINK1 gene in Miniature Schnauzers revealed 3 closely associated variants in healthy Miniature Schnauzers and Miniature Schnauzers with pancreatitis. These variants consisted of 2 missense mutations in the second exon (N20K and N25T) and a poly T insertion in the third intron that was near the boundary of exon 3 (IVS3+26-27ins(T)33-39,15_61dup11). Pancreatitis was significantly associated with homozygous alleles for these 3 variants in Miniature Schnauzers. In healthy dogs of other breeds, only the 2 exon variants were identified. CONCLUSIONS AND CLINICAL RELEVANCE: Variants of the SPINK1 gene may be associated with the development of pancreatitis in Miniature Schnauzers.

published proceedings

  • Am J Vet Res

altmetric score

  • 1.25

author list (cited authors)

  • Bishop, M. A., Xenoulis, P. G., Levinski, M. D., Suchodolski, J. S., & Steiner, J. M.

citation count

  • 20

complete list of authors

  • Bishop, Micah A||Xenoulis, Panagiotis G||Levinski, Melinda D||Suchodolski, Jan S||Steiner, Jörg M

publication date

  • May 2010