Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs. Academic Article uri icon

abstract

  • Adapter-modified Ussing chambers have been used for assessment of endoscopically obtained intestinal biopsies in humans. The aim of this study was to evaluate the feasibility of an adapter-modified Ussing chamber for assessment of intestinal transport physiology in endoscopically-obtained colonic biopsies from cats and dogs. Fifteen colonic biopsies from four cats and 13 colonic biopsies from four dogs were transferred into a modified Ussing chamber and sequentially exposed to several compounds. Baseline mean±SD conductance was measured. Changes of short circuit current (ΔIsc) were observed after exposure to glucose (number of feline biopsies that responded=0/number of canine biopsies that responded=4), phloridzin (n=0/n=7), histamine (n=5/n=12), serotonin (n=7/n=12), prostaglandin (n=5/n=7), forskolin (n=7/n=7), and ouabain (n=9/n=7). The adapter-modified Ussing chamber studied here enables investigation of transport physiology of endoscopically-obtained colonic biopsies from companion animals. However, we observed a large variability of results, suggesting that clinical use of this method is limited.

published proceedings

  • Res Vet Sci

altmetric score

  • 0.25

author list (cited authors)

  • Ruhnke, I., DeBiasio, J. V., Suchodolski, J. S., Newman, S. J., Musch, M. W., & Steiner, J. M.

citation count

  • 2

complete list of authors

  • Ruhnke, Isabelle||DeBiasio, John V||Suchodolski, Jan S||Newman, Shelley J||Musch, Mark W||Steiner, Jörg M

publication date

  • June 2012