Evaluation of the MYC_CANFA gene in Chinese Shar Peis with cobalamin deficiency. Academic Article uri icon

abstract

  • BACKGROUND: A recent genome-wide scan using the canine minimal screening set 2 (MSS-2) showed that cobalamin deficiency appears to be hereditary in Chinese Shar Peis and is linked to the microsatellite markers DTR13.6 and REN13N11 on canine chromosome 13. OBJECTIVE: The goal of this study was to evaluate the MYC_CANFA gene, which is the closest known gene with a distance of approximately 0.06 megabases (Mb) to the microsatellite marker DTR13.6, for any mutations in this breed. METHODS: Microsatellite markers (Myc and G15987) for genotyping and primers for sequencing were used to evaluate the MYC_CANFA gene. The genotype and gene sequence were compared between cobalamin-deficient Shar Peis, Shar Peis with normal serum cobalamin concentrations, and the DNA sequences published as part of the Ensemble Genomic map. RESULTS: Neither the microsatellite markers (Myc and G15987) nor the sequences of the MYC_CANFA gene showed a significant difference among both groups of Shar Peis and the published canine DNA sequence. CONCLUSIONS: The data presented here suggest that cobalamin deficiency in Shar Peis is not related to any mutations of the MYC_CANFA gene according to the genotyping and sequencing results in this study. Further investigations are warranted to find a potential genomic locus in proximity to DTR13.6 and REN13N11 that shows mutations in cobalamin-deficient Shar Peis.

published proceedings

  • Vet Clin Pathol

altmetric score

  • 1.1

author list (cited authors)

  • Grtzner, N., Bishop, M. A., Suchodolski, J. S., & Steiner, J. M.
  • Grützner, N., Bishop, M. A., Suchodolski, J. S., & Steiner, J. M
  • Grützner, N., Bishop, M. A., Suchodolski, J. S., & Steiner, J. M.

citation count

  • 1

complete list of authors

  • Grützner, Niels||Bishop, Micah A||Suchodolski, Jan S||Steiner, Jörg M

publication date

  • December 2012

publisher