Investigation of macrophage polarization using bone marrow derived macrophages. Academic Article uri icon


  • The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

published proceedings

  • Journal of visualized experiments : JoVE

altmetric score

  • 1.25

author list (cited authors)

  • Ying, W., Cheruku, P. S., Bazer, F. W., Safe, S. H., & Zhou, B.

citation count

  • 236

complete list of authors

  • Ying, W||Cheruku, PS||Bazer, FW||Safe, SH||Zhou, B

publication date

  • December 2013