Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents. Academic Article

abstract

• While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value.

authors

• Powell-Palm, Matthew

published proceedings

• Cryobiology

author list (cited authors)

• Powell-Palm, M. J., Zhang, Y., Aruda, J., & Rubinsky, B.

citation count

• 20

complete list of authors

• Powell-Palm, Matthew J||Zhang, Yanfang||Aruda, Justin||Rubinsky, Boris

publication date

• February 2019

publisher

• Elsevier  Publisher

published in

• Cryobiology  Journal

keywords

• Animals
• Cell Survival
• Cold Temperature
• Cryopreservation
• Cryoprotective Agents
• Freezing
• Islets Of Langerhans
• Isochoric Cryopreservation
• Models, Animal
• Organ Cryopreservation
• Osmosis
• Pancreatic Islets
• Rats
• Rats, Sprague-Dawley
• Thermodynamics

PubMed Central ID

• 30629949

Digital Object Identifier (DOI)

• 10.1016/j.cryobiol.2019.01.003

start page

• 130

end page

• 133

volume

• 86

URL

• http://dx.doi.org/10.1016/j.cryobiol.2019.01.003