Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents. Academic Article uri icon


  • While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value.

published proceedings

  • Cryobiology

author list (cited authors)

  • Powell-Palm, M. J., Zhang, Y., Aruda, J., & Rubinsky, B.

citation count

  • 20

complete list of authors

  • Powell-Palm, Matthew J||Zhang, Yanfang||Aruda, Justin||Rubinsky, Boris

publication date

  • February 2019