Multiplexed measurement of serum antibodies using an array biosensor.

abstract

• The array biosensor provides the capability for simultaneously measuring titers of antibody against multiple antigens. Human antibodies against four different targets, tetanus toxin, diphtheria toxin, staphylococcal enterotoxin B (SEB) and hepatitis B, were measured simultaneously in sera from eight different donors in a single assay and titers were determined. The assays could measure amounts of bound antibody as low as approximately 100 fg. Each individual serum exhibited a different pattern of reactivity against the four target antigens. Applications of this biosensor capability include monitoring for exposure to pathogens and for efficacy of vaccination.

authors

• Ligler, Frances

published proceedings

• Biosens Bioelectron

author list (cited authors)

• Moreno-Bondi, M. C., Taitt, C. R., Shriver-Lake, L. C., & Ligler, F. S.

citation count

• 46

complete list of authors

• Moreno-Bondi, Maria C||Taitt, Chris Rowe||Shriver-Lake, Lisa C||Ligler, Frances S

publication date

• April 2006

publisher

• Elsevier  Publisher

published in

• Biosensors and Bioelectronics  Journal

keywords

• Animals
• Antibodies, Bacterial
• Biosensing Techniques
• Diphtheria Toxin
• Enterotoxins
• Goats
• Humans
• Immunoglobulin G
• Protein Array Analysis
• Tetanus Toxin

PubMed Central ID

• 16434176

Digital Object Identifier (DOI)

• 10.1016/j.bios.2005.12.018

start page

• 1880

end page

• 1886

volume

• 21

issue

• 10

URL

• http://dx.doi.org/10.1016/j.bios.2005.12.018