Regeneration of immobilized antibodies on fiber optic probes. uri icon

abstract

  • The regeneration of antibodies covalently immobilized to an optical fibre surface was investigated by dissociation of the antibody-antigen complex with three different solvents: (a) an acidic solution (0.1 M glycine hydrochloride in 50% (v/v) ethylene glycol, pH 1.75), (b) a basic solution (0.05 M tetraethylamine in 50% (v/v) ethylene glycol, pH 11.0) and (c) 50% (v/v) ethanol in PBS. The fibres coated with polyclonal rabbit anti-goat antibody against a large protein retained 70% and 65% of the original signal after five consecutive regenerations with acidic and basic solvent systems, respectively. The fibres coated with monoclonal mouse anti-trinitrobenzene antibody specific for a small organic molecule, retained over 90% of the original signal when regenerated with basic and ethanol solutions. This study evaluated regeneration and reuse of antibody-coated fibre optic biosensors as a means of reducing routine laboratory analysis costs and time.

published proceedings

  • Biosens Bioelectron

altmetric score

  • 6

author list (cited authors)

  • Wijesuriya, D., Breslin, K., Anderson, G., Shriver-Lake, L., & Ligler, F. S.

citation count

  • 34

complete list of authors

  • Wijesuriya, D||Breslin, K||Anderson, G||Shriver-Lake, L||Ligler, FS

publication date

  • January 1994