Array biosensor: optical and fluidics systems. Academic Article uri icon

abstract

  • Optical and fluidics systems have been developed as central components for an automated array biosensor. Disposable planar waveguides are patterned with immobilized capture antibodies using a physically isolated patterning (PIP) method. The PIP method enables simultaneous deposition of several antibodies and completely circumvents cross-immobilization problems encountered with other array deposition processes. A multi-channel fluidics cell allows numerous assays to be performed on the patterned waveguide. The sensing arrays are optically interrogated using a diode laser with a tailored output to optimize coupling to and maximize excitation uniformity within the waveguide. A patterned cladding is employed to optically isolate the waveguide from perturbations induced by the permanently attached flow cells. Compact optics image the evanescently excited fluorescence onto a large area, cooled CCD array. The image data is processed and automated signal analysis corrects for local background and noise variations.

published proceedings

  • Biomed Microdevices

author list (cited authors)

  • Feldstein, M. J., Golden, J. P., Rowe, C. A., Maccraith, B. D., & Ligler, F. S.

citation count

  • 84

complete list of authors

  • Feldstein, MJ||Golden, JP||Rowe, CA||Maccraith, BD||Ligler, FS

publication date

  • June 1999