Detailed film cooling effectiveness distributions along a modeled turbine rotor blade under the combined effects of an upstream trailing edge unsteady wake with coolant ejection are presented using the pressure sensitive paint (PSP) mass transfer analogy method. The experiment is conducted in a low speed wind tunnel facility with a five blade linear cascade. The exit Reynolds number based on the axial chord is 370,000. Unsteady wakes and trailing edge coolant jets are produced by a spoked wheel-type wake generator with hollow rods equipped with several coolant ejections from holes. The coolant-to-mainstream density ratios for both the blade and trailing edge coolant ejection range from 1.5 to 2.0 for simulating realistic engine conditions. Blade blowing ratio studies are 0.5 and 1.0 on the suction surface and 1.0 and 2.0 on the pressure surface. The trailing edge jet blowing ratio and Strouhal numbers are 1.0 and 0.12, respectively. The results show that the unsteady wake reduces the overall film cooling effectiveness. However, the unsteady wake with trailing edge coolant ejection enhances the overall effectiveness. The results also show that the overall filming cooling effectiveness increases by using heavier coolant for trailing edge ejection and for blade surface film cooling.