Application of Fluorescence in Studying Therapeutic Enzymes. Chapter

abstract

• Fluorescence spectroscopy is one of the most important techniques in the study of therapeutic enzymes. The fluorescence phenomenon has been discovered and exploited for centuries, while therapeutic enzymes have been used in treatment of disease for only decades. This chapter provides a brief summary of the current applications of fluorescence methods in studying therapeutic enzymes to provide some insights on the selection of proper method tailored to the goal. First a brief introduction about therapeutic enzymes and history of fluorescence were provided, followed by discussions on how fluorescence was applied in the studies. Four popular fluorescence methods are discussed: fluorescence tracing, fluorescence resonance energy transfer (FRET), fluorescence quenching and fluorescence polarization. Selected application of the fluorescence methods in studying therapeutic enzymes are listed, and discussed in details in the following paragraphs.

authors

• Wei, Yinan

altmetric score

• 0.5

author list (cited authors)

• Wang, Z., Li, C., & Wei, Y.

citation count

• 2

complete list of authors

• Wang, Zhaoshuai||Li, Caihong||Wei, Yinan

Book Title

• Therapeutic Enzymes: Function and Clinical Implications

publication date

• January 2019

publisher

• Springer Nature  Publisher

published in

• Advances in Experimental Medicine and Biology  Journal

keywords

• ENZYMES
• FRET
• Fluorescence Polarization
• Fluorescence Quenching
• Fluorescence Resonance Energy Transfer
• Fluorescence Tracing
• Spectrometry, Fluorescence
• Staining And Labeling
• Therapeutic Enzyme

PubMed Central ID

• 31482496

Digital Object Identifier (DOI)

• 10.1007/978-981-13-7709-9_5

International Standard Book Number (ISBN) 13

• 9789811377082

start page

• 105

end page

• 114

volume

• 1148

URL

• http://dx.doi.org/10.1007/978-981-13-7709-9_5