Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+. Academic Article

abstract

• A novel ratiometric fluorescent peptidyl chemosensor (Dansyl-Cys-Pro-Gly-Cys-Trp-NH(2), D-P5) for metal ions detection has been synthesized via Fmoc solid-phase peptide synthesis. The chemosensor exhibited a high selectivity for Cd(2+) over other metal ions including competitive transition and Group I and II metal ions in neutral pH. The fluorescence emission intensity of D-P5 was significantly enhanced in the presence of Cd(2+) by fluorescent resonance energy transfer (FRET) and chelation enhanced fluorescence (CHEF) effects. The binding stoichiometry, detection limit, binding affinity, reversibility and pH sensitivity of the sensor for Cd(2+) were investigated.

authors

• Ma, Guolin

published proceedings

• Bioorg Med Chem Lett

author list (cited authors)

• Li, Y., Li, L., Pu, X., Ma, G., Wang, E., Kong, J., Liu, Z., & Liu, Y.

citation count

• 56

complete list of authors

• Li, Yan||Li, Lianzhi||Pu, Xuewei||Ma, Guolin||Wang, Erqiong||Kong, Jinming||Liu, Zhipeng||Liu, Yangzhong

publication date

• June 2012

publisher

• Elsevier  Publisher

published in

• Bioorganic and Medicinal Chemistry Letters  Journal

keywords

• Cadmium
• Cations, Divalent
• FLUORESCENCE
• Fluorescence Resonance Energy Transfer
• Fluorescent Dyes
• Hydrogen-Ion Concentration
• Molecular Structure
• Peptides
• Phosphatidylcholines
• Protein Binding
• Sensitivity And Specificity
• Solid-phase Synthesis Techniques
• Water Pollutants, Chemical

PubMed Central ID

• 22579421

Digital Object Identifier (DOI)

• 10.1016/j.bmcl.2012.04.088

start page

• 4014

end page

• 4017

volume

• 22

issue

• 12

URL

• http://dx.doi.org/10.1016/j.bmcl.2012.04.088