Differentially expressed gene profiles between multidrug resistant gastric adenocarcinoma cells and their parental cells. Academic Article

abstract

• To better understand the molecular mechanisms of multidrug resistance (MDR) of human cancers, we isolated differentially expressed genes from drug-resistant human gastric adenocarcinoma cell lines using a polymerase chain reaction-based subtractive hybridization technique. Sixty three genes were identified to be differentially expressed in the drug-resistant human gastric adenocarcinoma cell lines. Among the 63 up-regulated genes, 27 were known genes, of which two have been reported to be associated with MDR. The remaining ones were undefined genes or expressed sequenced tags. The results suggest that this strategy is efficient for large-scale cloning of differentially expressed genes in drug-resistant cells. Further characterization of these genes will shed more light on the understanding of molecular mechanisms of MDR of human cancer cells.

authors

• Liu, Fei

published proceedings

• Cancer Lett

author list (cited authors)

• Zhao, Y., You, H., Liu, F., An, H., Shi, Y., Yu, Q., & Fan, D.

citation count

• 102

complete list of authors

• Zhao, Yanqiu||You, Han||Liu, Fei||An, Huazhang||Shi, Yongquan||Yu, Qiang||Fan, Daiming

publication date

• November 2002

publisher

• Elsevier  Publisher

published in

• Cancer Letters  Journal

keywords

• Adenocarcinoma
• Cloning, Molecular
• Doxorubicin
• Drug Resistance, Multiple
• Drug Resistance, Neoplasm
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Neoplasm Proteins
• Polymerase Chain Reaction
• Stomach Neoplasms
• Subtraction Technique
• Tumor Cells, Cultured
• Vincristine

PubMed Central ID

• 12169395

Digital Object Identifier (DOI)

• 10.1016/s0304-3835(02)00264-1

start page

• 211

end page

• 218

volume

• 185

issue

• 2

URL

• http://dx.doi.org/10.1016/s0304-3835(02)00264-1