Analysis of Lymphatic Vessel Formation by Whole-Mount Immunofluorescence Staining. Chapter

abstract

• Pathological alterations of lymphatic structure and function interfere with lymph transport, resulting in a wide range of clinical disorders that include edema, tissue inflammation, and metabolic syndromes. Mesentery contains abundant lymphatic vessels and plays an important role in transporting absorbed lipid from the intestine. In this manuscript, we describe a whole-mount staining method on isolated mouse mesentery with VEGFR3, Prox1, and Lyve1 antibodies to visualize the morphology of lymphatic vessels.

authors

• Muthuchamy, Mariappan
• Peng, Xu
• Zawieja, David

author list (cited authors)

• Wang, J., Dong, Y., Muthuchamy, M., Zawieja, D. C., & Peng, X. u.

citation count

• 1

complete list of authors

• Wang, Jian||Dong, Yuwei||Muthuchamy, Mariappan||Zawieja, David C||Peng, Xu

Book Title

• Cardiovascular Development

publication date

• January 2021

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Embryo, Mammalian
• Female
• Homeodomain Proteins
• Immunofluorescence Staining
• Lymphangiogenesis
• Lymphatic Endothelial Cells
• Lymphatic Vessels
• Mesentery
• Mice
• Microscopy, Fluorescence
• Staining And Labeling
• Tumor Suppressor Proteins
• Vascular Endothelial Growth Factors
• Vesicular Transport Proteins
• Whole-mount Staining

PubMed Central ID

• 34331253

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-1480-8_17

International Standard Book Number (ISBN) 13

• 9781071614792

start page

• 153

end page

• 159

volume

• 2319

URL

• http://dx.doi.org/10.1007/978-1-0716-1480-8_17