Analysis of Lymphatic Vessel Formation by Whole-Mount Immunofluorescence Staining. Chapter uri icon

abstract

  • Pathological alterations of lymphatic structure and function interfere with lymph transport, resulting in a wide range of clinical disorders that include edema, tissue inflammation, and metabolic syndromes. Mesentery contains abundant lymphatic vessels and plays an important role in transporting absorbed lipid from the intestine. In this manuscript, we describe a whole-mount staining method on isolated mouse mesentery with VEGFR3, Prox1, and Lyve1 antibodies to visualize the morphology of lymphatic vessels.

author list (cited authors)

  • Wang, J., Dong, Y., Muthuchamy, M., Zawieja, D. C., & Peng, X. u.

complete list of authors

  • Wang, Jian||Dong, Yuwei||Muthuchamy, Mariappan||Zawieja, David C||Peng, Xu

Book Title

  • Methods in Molecular Biology

publication date

  • July 2021