Imaging Epidermal Cell Rearrangement in the C. elegans Embryo. Chapter uri icon

abstract

  • The Caenorhabditis elegans embryo is well suited for analysis of directed cell rearrangement via modern microscopy, due to its simple organization, short generation time, transparency, invariant lineage, and the ability to generate engineered embryos expressing various fluorescent proteins. This chapter provides an overview of routine microscopy techniques for imaging dorsal intercalation, a convergent extension-like morphogenetic movement in the embryonic epidermis of C. elegans, including making agar mounts, low-cost four-dimensional (4D) Nomarski microscopy, laser microsurgery, and 4D fluorescence microscopy using actin and junctional fusion proteins, as well as tissue-specific promoters useful for studying dorsal intercalation.

author list (cited authors)

  • Hardin, J., Serre, J., King, R., Walck-Shannon, E., & Reiner, D.

complete list of authors

  • Hardin, Jeff||Serre, Joel||King, Ryan||Walck-Shannon, Elise||Reiner, David

Book Title

  • Cell Polarity Signaling

publication date

  • January 1, 2022 11:11 AM