Imaging Epidermal Cell Rearrangement in the C. elegans Embryo. Chapter

abstract

• The Caenorhabditis elegans embryo is well suited for analysis of directed cell rearrangement via modern microscopy, due to its simple organization, short generation time, transparency, invariant lineage, and the ability to generate engineered embryos expressing various fluorescent proteins. This chapter provides an overview of routine microscopy techniques for imaging dorsal intercalation, a convergent extension-like morphogenetic movement in the embryonic epidermis of C. elegans, including making agar mounts, low-cost four-dimensional (4D) Nomarski microscopy, laser microsurgery, and 4D fluorescence microscopy using actin and junctional fusion proteins, as well as tissue-specific promoters useful for studying dorsal intercalation.

authors

• Reiner, David

altmetric score

• 0.75

author list (cited authors)

• Hardin, J., Serre, J., King, R., Walck-Shannon, E., & Reiner, D.

citation count

• 1

complete list of authors

• Hardin, Jeff||Serre, Joel||King, Ryan||Walck-Shannon, Elise||Reiner, David

Book Title

• Cell Polarity Signaling

publication date

• January 2022

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• C. Elegans
• Caenorhabditis Elegans
• Caenorhabditis Elegans Proteins
• Cell Intercalation
• Convergent Extension
• Embryo, Nonmammalian
• Epidermal Cells
• Epidermis
• Microscopy, Fluorescence
• Morphogenesis

PubMed Central ID

• 35147953

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-2035-9_22

International Standard Book Number (ISBN) 13

• 9781071620342

start page

• 345

end page

• 376

volume

• 2438

URL

• http://dx.doi.org/10.1007/978-1-0716-2035-9_22