Bioavailability and metabolomic targets of sulforaphane in humans (1036.2)
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abstract
Sulforaphane (SFN), an isothiocyanate derived from cruciferous vegetables, has many potential health benefits. Yet, there is limited knowledge about its bioavailability and biological functions in humans. Crucifers contain glucoraphanin (GFN), which is hydrolyzed to SFN by myrosinase (MYR). Study objectives were to determine SFN bioavailability and examine metabolomic profiles in plasma following consumption of a MYRtreated broccoli sprout extract (BSE) supplement and broccoli sprouts. We have previously shown a 7fold decrease in SFN bioavailability from GFN supplements with inactivated MYR than from broccoli sprouts, suggesting MYR activity impacts SFN bioavailability. SFN bioavailability from BSEs was improved over MYRinactivated GFN supplements but was ~3 times lower than sprouts. Highthroughput metabolomic analysis revealed alterations in plasma metabolites following SFN consumption. Unique metabolomic profiles following BSE and sprout consumption suggested differential metabolic responses from the supplemental and wholefood forms of SFN. BSE and sprout consumption was associated with unique biochemical targets, and several common targets of the two SFN forms were altered in opposite directions. This research provides important information for conducting SFN supplementation studies to understand SFNs role in disease prevention.Grant Funding Source: Supported by R01CA122906, P01CA090890, P30 ES000210
