Sulforaphane alters the expression of long intragenic noncoding RNAs that are dysregulated in prostate cancer cells (644.10)
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abstract
The dysregulation of long intergenic noncoding RNAs (lincRNAs) has become an emerging factor in cancer development. Dietary chemopreventive compounds, such as sulforaphane (SFN) from cruciferous vegetables, can alter epigenetic targets for cancer prevention but the impact of diet on lincRNA expression is unknown. Normal prostate epithelial cells and prostate cancer cells were treated with 15 M SFN to test the hypothesis that SFN alters the expression of lincRNAs. RNAsequencing revealed that hundreds of lincRNAs were differentially expressed in prostate cancer cells relative to normal prostate epithelial cells. SFN treatment significantly altered the expression of ~70 lincRNAs in each cell type. The SFNinduced response differed depending on cell line and was dynamic over time. In prostate cancer cells, SFN treatment reversed the aberrant expression of some lincRNAs. Preliminary guilt by association work showed that lincRNA's altered by SFN treatment correlate with genes that regulate angiogenesis, cell proliferation, migration, differentiation, apoptosis, and the glutathione metabolic process. Studies evaluating the functional role of these lincRNAs in prostate cancer development are ongoing. Ultimately, this data revealed a novel mechanism by which SFN impacts prostate cancer that may be leveraged to develop future successful prostate cancer prevention strategies. Funding: P01CA090890Grant Funding Source: Supported by P01CA090890
