Optimum drift velocity for single molecule fluorescence bursts in micro/nano-fluidic channels Academic Article

abstract

• Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This paper is a sequel to an earlier one of ours [L. L. Kish, Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity that minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications. 2012 American Institute of Physics.

authors

• Kameoka, Jun
• Kish, Laszlo

published proceedings

• APPLIED PHYSICS LETTERS

altmetric score

• 1.1

author list (cited authors)

• Kish, L. L., Kameoka, J., Granqvist, C. G., & Kish, L. B.

citation count

• 0

complete list of authors

• Kish, Lazar L||Kameoka, Jun||Granqvist, Claes G||Kish, Laszlo B

publication date

• January 2012

publisher

• AIP Publishing  Publisher

published in

• Applied Physics Letters  Journal

keywords

• Physics.gen-ph
• Physics.ins-det

Digital Object Identifier (DOI)

• 10.1063/1.4739471

start page

• 043120

end page

• 043120

volume

• 101

issue

• 4

URL

• http://dx.doi.org/10.1063/1.4739471