Quantitative analysis of alkaloidal constituents in imported fire ants by gas chromatography. Academic Article

abstract

• A method based on silica gel chromatography and GC-MS/GC-FID analyses was developed for the quantitation of alkaloidal compounds in imported fire ants, Solenopsis richteri, S. invicta, and their hybrid found in the southern United States. The cis and trans alkaloids from fire ant body extracts were successfully separated by silica gel chromatography, identified by GC-MS, and quantitated by GC-FID. Piperideine compounds were eluted together with the cis and trans piperidines, but were well-resolved on a nonpolar GC column. Eight pairs of piperidine isomers and 12 piperideines were quantitated. The ratios of trans alkaloids to corresponding cis isomers ranged from 87 to 378:1 in S. invicta and were significantly higher than in S. richteri and hybrid ants. The results were discussed in relation to the evolution of fire ant venom alkaloids and their role as host location cues for parasitic Pseudacteon phorid flies.

authors

• Fadamiro, Henry

published proceedings

• J Agric Food Chem

author list (cited authors)

• Yu, Y., Wei, H., Fadamiro, H. Y., & Chen, L. i.

citation count

• 11

complete list of authors

• Yu, Yu-Ting||Wei, Hong-Yi||Fadamiro, Henry Y||Chen, Li

publication date

• June 2014

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of Agricultural and Food Chemistry  Journal

keywords

• Alkaloids
• Animals
• Ants
• Chromatography, Gas
• Isomerism
• Molecular Structure
• Piperidines
• Venoms

PubMed Central ID

• 24873743

Digital Object Identifier (DOI)

• 10.1021/jf501423y

start page

• 5907

end page

• 5915

volume

• 62

issue

• 25

URL

• http://dx.doi.org/10.1021/jf501423y