Mutagenic and apoptotic effects of white mold (Sclerotinia sclerotiorum) in vivo and in vitro (824.6)
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abstract
Various common foods, including beans, soybeans, peas, and potatoes, are often contaminated by soil fungus called white mold, Sclerotinia sclerotiorum. To data, little information is available regarding the toxicological effects. This work aimed to determine the apoptotic and mutagenic potential of aqueous extract of Sclerotinia sclerotiorum (AESS) in vivo and in vitro. Chemical analysis showed that the extract was rich in oxygenated monoterpenes, such as camphor. Swiss mice were fed 10, 100 and 1000mg/kg bodyweight of AESS for 15 days. The comet assay was performed in peripheral blood cells and the number of micronucleated polychromatic erythrocytes (MNPCEs) was determined in bone marrow cells. In addition, HT29 and CCD18 cell lines were treated with 0.005, 0.01 and 0.02ppm of AESS for performing cell viability assay and RTPCR. In results, the extract had mutagenic effects in the micronucleus test, by increasing the number of MNPCEs up to 439%. However, the extract did not show any DNAdamage in the comet assay. In vitro, the extract demonstrated proapoptotic effects through modulation of gene expressions associated with p53mediated apoptosis pathway in both cancer and noncancer colon cells within a low concentration range. In conclusion, the AESS did not cause DNA damage in the comet assay, but potential mutagenic and cytotoxic activities within a low concentration range should be further investigated.
