Mango polyphenols reduce inflammation in MDA-MB231 breast cancer cell and targets MicroRNA-21 Academic Article uri icon

abstract

  • Mango polyphenols have received some attention based on their antioxidant, anticarcinogenic and antiinflammatory properties. Our objective was to determine the antiinflammatory and cytotoxic properties of mango polyphenols (MP) in MCF12A noncancer breast fibroblasts and MDAMB231 breast cancer cells. mRNA, miRNA and protein expression of MDAMB231 and MCF12A cells were analyzed by real time PCR and Western blotting.Results show that MP (5mg of gallic acid equivalents/L (GAE/L)) decreased the proliferation of MB231 breast cancer cells by 90%; but not of MCF12A cells. In MB231 breast cancer cells, MP downregulated the expression of NFB (to 0.6fold of the control), VEGF (0.5fold), TNF (0.8fold), survivin (0.5fold) and Bcl2 (0.5fold). In MCF12A breast cells, MP downregulated TNFinduced expression of IL6 (0.8fold), IL1 (0.6fold) and NFKb (0.4 fold). miRNA 21 is upregulated in breast cancer and targets Bcl2 which is involved in apoptosis. MP reduced the expression the miR21 in a dose dependent manner and these findings were confirmed using the specific antagomir for miR21, where it reversed the effect of the polyphenolic treatment in MB231 cells.These results indicate that the cytotoxic effects of mango polyphenols are specific to cancer cells where inflammation was reduced in both, cancer and noncancer cells, seemingly through the involvement of miR21.Grant Funding Source: Research Assistanship

published proceedings

  • FASEB JOURNAL

author list (cited authors)

  • Arbizu, S., Krenek, K., & Mertens-Talcott, S.

citation count

  • 3

complete list of authors

  • Arbizu, Shirley||Krenek, Kimberly||Mertens‐Talcott, Susanne

publication date

  • April 2013

publisher