Effect of Nonionic Surfactant on Association/Dissociation Transition of DNA-Functionalized Colloids. Academic Article

abstract

• We report the effect of nonionic surfactants (Pluronics F127 and F88) on the melting transition of micron-sized colloids confined in two dimensions, mediated by complementary single-stranded DNA as a function of the surfactant concentration. Micron-sized silica particles were functionalized with single-stranded DNA using cyanuric chloride chemistry. The existence of covalently linked DNA on particles was confirmed by fluorescence spectroscopy. The nonionic surfactant not only plays a significant role in stabilization of particles, with minimization of nonspecific binding, but also impacts the melting temperature, which increases as a function of the nonionic surfactant concentration. These results suggest that the melting transition for DNA-mediated assembly is sensitive to commonly used additives in laboratory buffers, and that these common solution components may be exploited as a facile and independent handle for tuning the melting temperature and, thus, the assembly and possibly crystallization within these systems.

authors

• Mittal, Jeetain

published proceedings

• Langmuir

altmetric score

• 0.25

author list (cited authors)

• Song, M., Ding, Y., Snyder, M. A., & Mittal, J.

citation count

• 7

complete list of authors

• Song, Minseok||Ding, Yajun||Snyder, Mark A||Mittal, Jeetain

publication date

• October 2016

publisher

• American Chemical Society (ACS)  Publisher

published in

• Langmuir  Journal

keywords

• Base Sequence
• Colloids
• DNA, Single-Stranded
• Micelles
• Nucleic Acid Hybridization
• Particle Size
• Phase Transition
• Poloxamer
• Silicon Dioxide
• Surface-Active Agents
• Transition Temperature

PubMed Central ID

• 27595803

Digital Object Identifier (DOI)

• 10.1021/acs.langmuir.6b02096

start page

• 10017

end page

• 10025

volume

• 32

issue

• 39

URL

• http://dx.doi.org/10.1021/acs.langmuir.6b02096