Spray cooling is one of the most promising technologies in applications which require large heat removal capacity in very small areas. Previous experimental studies have suggested that one of the main mechanisms of heat removal in spray cooling is forced convection with strong mixing due to droplet impingement. These mechanisms have not been completely understood mainly due to the large number of physical variables, and the inability to modulate and control variables such as droplet frequency and size. Our approach consists of minimizing the number of experimental variables by controlling variables such as droplet direction, velocity and diameter. An experimental study of single and multiple droplet impingements using HFE 7100 as the cooling fluid under constant heat flux conditions is presented. A monosized droplet train is produced using a piezoelectric droplet generator with the ability to adjust droplet frequency, diameter and velocity. In this study, heaters consisting of a layer of Indium Tin Oxide (ITO) as heating element, and silicon substrates are used. Film morphology was characterized using a Laser Induced Fluorescence (LIF) technique with a focus on the droplet impact zone by measuring variables such as film thickness and diameter of the impact zone. Infrared thermography was used to measure surface temperature at the liquid-solid interface. The IR thermography technique was also used to characterize temperature gradients at the droplet impact zone. The results and effects of droplet frequency, fluid flow rate, and fluid temperature on heat flux are also presented.