Regulatory mutants and transcriptional control of the Serratia marcescens extracellular nuclease gene. Academic Article

abstract

• The extracellular nuclease of Serratia marcescens is regulated in a complex fashion. Unlike most catabolic enzymes, it appears not to be substrate regulated. However we have shown it to be regulated by an SOS-like system in S. marcescens. Additionally nuclease expression is regulated in a growth-phase-dependent manner. In this work we demonstrate that growth-phase-dependent regulation is at the transcriptional level. The putative LexA-binding site which mediates SOS regulation is shown to act as an operator site in vivo. The boundaries of a minimal promoter, still regulated by growth phase and SOS regulation, are defined along with the transcriptional start site. However, a region upstream of the nuclease promoter is shown to enhance significantly the expression of nuclease.

authors

• Benedik, Michael

published proceedings

• Mol Microbiol

author list (cited authors)

• Chen, Y. C., Shipley, G. L., Ball, T. K., & Benedik, M. J.

citation count

• 22

complete list of authors

• Chen, YC||Shipley, GL||Ball, TK||Benedik, MJ

publication date

• March 1992

publisher

• Wiley  Publisher

published in

• Molecular Microbiology  Journal

keywords

• Amino Acid Sequence
• Base Sequence
• Cell Cycle
• Chromosome Mapping
• DNA, Bacterial
• Endodeoxyribonucleases
• Endonucleases
• Endoribonucleases
• Gene Expression Regulation, Bacterial
• Gene Expression Regulation, Enzymologic
• Molecular Sequence Data
• Mutation
• Promoter Regions, Genetic
• RNA, Bacterial
• Regulatory Sequences, Nucleic Acid
• Serratia Marcescens
• Sos Response, Genetics
• Transcription, Genetic

PubMed Central ID

• 1372678

Digital Object Identifier (DOI)

• 10.1111/j.1365-2958.1992.tb01512.x

start page

• 643

end page

• 651

volume

• 6

issue

• 5