Inhibition of Serratia marcescens nuclease secretion by a truncated nuclease peptide. Academic Article

abstract

• The production of extracellular nuclease (Nuc) from the Serratia marcescens nucA chromosomal locus is inhibited in cells producing the N-terminal portion of Nuc from a multicopy plasmid. This inhibition in trans is not at the level of nucA expression, but rather at the level of secretion of the Nuc protein. Production of the periplasmic protein beta-lactamase (Bla) does not inhibit Nuc production unless fused to the nucA signal peptide and expressed from nucAp. Inhibition by either the truncated Nuc peptide (delta Nuc) or a Bla fusion protein is promoter specific and observed when expressed from nucAp; little inhibition is observed when the same protein is expressed from the lacZpo promoter-operator. This promoter specificity is also true for the secretion of Nuc itself.

authors

• Benedik, Michael

published proceedings

• Gene

author list (cited authors)

• Chen, Y. C., Suh, Y., Riise, E., Kartman, B., Jin, S., & Benedik, M. J.

citation count

• 3

complete list of authors

• Chen, YC||Suh, Y||Riise, E||Kartman, B||Jin, S||Benedik, MJ

publication date

• January 1996

publisher

• Elsevier  Publisher

published in

• Gene  Journal

keywords

• Endodeoxyribonucleases
• Endoribonucleases
• Peptide Fragments
• Promoter Regions, Genetic
• Protein Biosynthesis
• Sequence Deletion
• Serratia Marcescens
• Transcription, Genetic

PubMed Central ID

• 8654998

Digital Object Identifier (DOI)

• 10.1016/0378-1119(96)00187-4

start page

• 9

end page

• 16

volume

• 172

issue

• 1

URL

• http://dx.doi.org/10.1016/0378-1119(96)00187-4