Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism. Academic Article uri icon


  • Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclease has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.

published proceedings

  • Biochemistry (Mosc)

author list (cited authors)

  • Filimonova, M. N., Gubskaya, V. P., Nuretdinov, I. A., Benedik, M. J., Bogomolnaya, L. M., Andreeva, M. A., & Leshchinskaya, I. B.

citation count

  • 5

complete list of authors

  • Filimonova, MN||Gubskaya, VP||Nuretdinov, IA||Benedik, MJ||Bogomolnaya, LM||Andreeva, MA||Leshchinskaya, IB

publication date

  • September 1997