[Polydispersity of Serratia marcescens nuclease at optimal pH values]. Academic Article

abstract

• Treatment with dimethyl suberimidate, a cross-linking bifunctional agent, showed that Sm1 and Sm2 nucleases of Serratia marcescens B10M1 are polydisperse in solution and consist of monomers and dimers at the level of pH optimal for the enzyme activity. The data suggest that nucleases from the strain B10M1 and any other strain are polydisperse at pH optimum if their amino acid sequences are identical.

authors

• Benedik, Michael

published proceedings

• Prikl Biokhim Mikrobiol

author list (cited authors)

• Filimonova, M. N., Benedik, M. J., Urazov, N. G., & Leshchinskaia, I. B.

citation count

• 4

complete list of authors

• Filimonova, MN||Benedik, MJ||Urazov, NG||Leshchinskaia, IB

publication date

• January 1999

published in

• n0555-1099ISSN  Journal

keywords

• Amino Acid Sequence
• Cross-Linking Reagents
• Dimerization
• Dimethyl Suberimidate
• Electrophoresis, Polyacrylamide Gel
• Endodeoxyribonucleases
• Endoribonucleases
• Hydrogen-Ion Concentration
• Molecular Sequence Data

PubMed Central ID

• 10330672

start page

• 20

end page

• 24

volume

• 35

issue

• 1