Mutant analysis shows that alanine racemases from Pseudomonas aeruginosa and Escherichia coli are dimeric. Academic Article uri icon


  • Alanine racemases are ubiquitous prokaryotic enzymes providing the essential peptidoglycan precursor D-alanine. We present evidence that the enzymes from Pseudomonas aeruginosa and Escherichia coli function exclusively as homodimers. Moreover, we demonstrate that expression of a K35A Y235A double mutation of dadX in E. coli suppresses bacterial growth in a dominant negative fashion.

published proceedings

  • J Bacteriol

altmetric score

  • 6

author list (cited authors)

  • Strych, U., & Benedik, M. J.

citation count

  • 36

complete list of authors

  • Strych, Ulrich||Benedik, Michael J

publication date

  • August 2002