Endocrine disrupting chemicals differentially alter intranuclear dynamics and transcriptional activation of estrogen receptor-. Academic Article uri icon


  • Transcription is a highly regulated sequence of stochastic processes utilizing many regulators, including nuclear receptors (NR) that respond to stimuli. Endocrine disrupting chemicals (EDCs) in the environment can compete with natural ligands for nuclear receptors to alter transcription. As nuclear dynamics can be tightly linked to transcription, it is important to determine how EDCs affect NR mobility. We use an EPA-assembled set of 45 estrogen receptor- (ER) ligands and EDCs in our engineered PRL-Array model to characterize their effect upon transcription using fluorescence in situ hybridization and fluorescence recovery after photobleaching (FRAP). We identified 36 compounds that target ER-GFP to a transcriptionally active, visible locus. Using a novel method for multi-region FRAP analysis we find a strong negative correlation between ER mobility and inverse agonists. Our findings indicate that ER mobility is not solely tied to transcription but affected highly by the chemical class binding the receptor.

published proceedings

  • iScience

altmetric score

  • 38.08

author list (cited authors)

  • Bolt, M. J., Singh, P., Obkirchner, C. E., Powell, R. T., Mancini, M. G., Szafran, A. T., Stossi, F., & Mancini, M. A.

citation count

  • 0

complete list of authors

  • Bolt, Michael J||Singh, Pankaj||Obkirchner, Caroline E||Powell, Reid T||Mancini, Maureen G||Szafran, Adam T||Stossi, Fabio||Mancini, Michael A

publication date

  • November 2021