Raman spectroscopy-based diagnostics of water deficit and salinity stresses in two accessions of peanut. Academic Article uri icon


  • Water deficit and salinity are two major abiotic stresses that have tremendous effect on crop yield worldwide. Timely identification of these stresses can help limit associated yield loss. Confirmatory detection and identification of water deficit stress can also enable proper irrigation management. Traditionally, unmanned aerial vehicle (UAV)-based imaging and satellite-based imaging, together with visual field observation, are used for diagnostics of such stresses. However, these approaches can only detect salinity and water deficit stress at the symptomatic stage. Raman spectroscopy (RS) is a noninvasive and nondestructive technique that can identify and detect plant biotic and abiotic stress. In this study, we investigated accuracy of Raman-based diagnostics of water deficit and salinity stresses on two greenhouse-grown peanut accessions: tolerant and susceptible to water deficit. Plants were grown for 76days prior to application of the water deficit and salinity stresses. Water deficit treatments received no irrigation for 5days, and salinity treatments received 1.0L of 240-mM salt water per day for the duration of 5-day sampling. Every day after the stress was imposed, plant leaves were collected and immediately analyzed by a hand-held Raman spectrometer. RS and chemometrics could identify control and stressed (either water deficit or salinity) susceptible plants with 95% and 80% accuracy just 1day after treatment. Water deficit and salinity stressed plants could be differentiated from each other with 87% and 86% accuracy, respectively. In the tolerant accessions at the same timepoint, the identification accuracies were 66%, 65%, 67%, and 69% for control, combined stresses, water deficit, and salinity stresses, respectively. The high selectivity and specificity for presymptomatic identification of abiotic stresses in the susceptible line provide evidence for the potential of Raman-based surveillance in commercial-scale agriculture and digital farming.

published proceedings

  • Plant Direct

altmetric score

  • 2.5

author list (cited authors)

  • Morey, R., Farber, C., McCutchen, B., Burow, M. D., Simpson, C., Kurouski, D., & Cason, J.

citation count

  • 2

complete list of authors

  • Morey, Rohini||Farber, Charles||McCutchen, Bill||Burow, Mark D||Simpson, Charles||Kurouski, Dmitry||Cason, John

publication date

  • August 2021