Isolation of Lymphatic Muscle Cells (LMCs ) from Rat Mesentery. Chapter

abstract

• Lymphatic muscle cells (LMCs), with unique characteristics resembling a combination of both cardiac and smooth muscle cells, play an essential role in the spontaneous contraction of the lymphatic vessels to pump fluid forward. However, our understanding of the more detailed molecular phenotypes of LMCs is limited. Here, we described a method to isolate the LMCs from rat mesentery and then culture the cells in vitro, which will serve a lot more molecular biology study of LMCs and significantly improve our knowledge about the unique characteristics of LMCs.

authors

• Chakraborty, Sanjukta
• Muthuchamy, Mariappan
• Zawieja, David

author list (cited authors)

• Zhang, X., Chakraborty, S., Muthuchamy, M., & Zawieja, D. C.

citation count

• 1

complete list of authors

• Zhang, Xueyang||Chakraborty, Sanjukta||Muthuchamy, Mariappan||Zawieja, David C

Book Title

• Cardiovascular Development

publication date

• January 2021

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Cell Culture Techniques
• Cell Separation
• Dissection
• Fluorescent Antibody Technique
• Isolation
• Lymphatic Muscle Cells (lmcs)
• Mesentery
• Muscle Cells
• RAT MESENTERY
• Rats

PubMed Central ID

• 34331251

Digital Object Identifier (DOI)

• 10.1007/978-1-0716-1480-8_15

International Standard Book Number (ISBN) 13

• 9781071614792

start page

• 137

end page

• 141

volume

• 2319

URL

• http://dx.doi.org/10.1007/978-1-0716-1480-8_15