The Clostridium difficile proline racemase is not essential for early logarithmic growth and infection. Academic Article

abstract

• Proline racemase (PrdF), which is important for energy metabolism via the Stickland pathway and is unique to certain clostridia, was investigated as a potential anti-Clostridium difficile target by examining its effects on the growth and virulence of C. difficile. Inactivation of PrdF by insertional mutagenesis did not affect early logarithmic growth but only attenuated growth in the mid- and late logarithmic phases. There was no effect on virulence in vivo, suggesting that PrdF is also not required for C. difficile infection. These findings indicate that PrdF as well as other enzymes encoded by the proline reductase operon are all nonessential and are unsuitable targets for anti-C. difficile drug discovery.

authors

• Hurdle, Julian

published proceedings

• Can J Microbiol

altmetric score

• 4.85

author list (cited authors)

• Wu, X., & Hurdle, J. G.

citation count

• 13

complete list of authors

• Wu, Xiaoqian||Hurdle, Julian G

publication date

• April 2014

publisher

• Canadian Science Publishing  Publisher

published in

• Canadian Journal of Microbiology  Journal

keywords

• Amino Acid Isomerases
• Animals
• Clostridioides Difficile
• Computational Biology
• Cricetinae
• Drug Delivery Systems
• Enterocolitis, Pseudomembranous
• Humans
• Mutagenesis, Insertional
• Operon
• Virulence

PubMed Central ID

• 24693984

Digital Object Identifier (DOI)

• 10.1139/cjm-2013-0903

start page

• 251

end page

• 254

volume

• 60

issue

• 4

URL

• http://dx.doi.org/10.1139/cjm-2013-0903