Validation of a Mathematical Model of Gene Transcription in Aggregated Cellular Systems Application to L1 Retrotransposition Academic Article uri icon

abstract

  • We present a methodology aimed at partial validation and accuracy-precision assessment of a mathematical model of gene transcription at the cellular level. The method is based on the analysis of time-series measurements aggregated over a large number of cells. Such measurements are typically obtained via reverse transcriptase-polymerase chain reaction (RT-PCR) experiments. The validation procedure presented herein uses as an example data on L1 retrotransposon gene in HeLa cells. The procedure compares model predicted values with the RT-PCR data for L1 by means of the standard Bayesian statistical techniques with the help of modern Markov-Chain Monte-Carlo methodology.

author list (cited authors)

  • Rempala, G. A., Ramos, K. S., Kalbfleisch, T., & Teneng, I.

citation count

  • 11

publication date

  • April 2007