Subunit-specific polyclonal antibody targeting human 1 GABA(C) receptor. Academic Article uri icon

abstract

  • The GABA(C) receptor, a postsynaptic membrane receptor expressed prominently in the retina, is a ligand-gated ion channel that consists of a combination of subunits. We report characterization of a novel guinea pig polyclonal antibody, termed GABA(C) Ab N-14, directed against a 14-mer peptide (N-14) of the extracellular domain of the human 1 subunit. The antibody exhibits high sensitivity for N-14 by ELISA. In Western blots, GABA(C) Ab N-14 shows reactivity with the 1 subunit of preparations obtained from 1 GABA(C)-expressing neuroblastoma cells, Xenopus oocytes, and mammalian retina and brain. Flow cytometry reveals a rightward shift in mean fluorescence intensity of GABA(C)-expressing neuroblastoma cells probed with GABA(C) Ab N-14. Immunostaining of neuroblastoma cells and oocytes with GABA(C) Ab N-14 yields fluorescence only with GABA(C)-expressing cells. Antibody binding has no effect on GABA-elicited membrane current responses. Immunostaining of human retinal sections shows preferential staining within the inner plexiform layer. GABA(C) Ab N-14 appears well suited for investigative studies of GABA(C) 1 subunit in retina and other neural tissues.

published proceedings

  • Exp Eye Res

altmetric score

  • 3

author list (cited authors)

  • Gussin, H. A., Khasawneh, F. T., Xie, A. n., Feng, F., Memic, A., Qian, H., Le Breton, G. C., & Pepperberg, D. R.

citation count

  • 1

complete list of authors

  • Gussin, Hélène A||Khasawneh, Fadi T||Xie, An||Feng, Feng||Memic, Adnan||Qian, Haohua||Le Breton, Guy C||Pepperberg, David R

publication date

  • January 2011