Pomegranate Polyphenolics reduce inflammation in Intestinal Colitis - Potential Involvement of the miR-145/p70S6K/HIF1 alpha Pathway Conference Paper uri icon

abstract

  • Pomegranate polyphenolics have previously been demonstrated effective in the prevention of inflammation and carcinogenesis. To determine the antiinflammatory effects and possible mechanisms of pomegranate polyphenolics in colitis, the mRNA and protein levels were assessed in colonic mucosa by RTPCR, western blotting and multiplex bead assay.Compared to the control, pomegranate suppressed cell proliferation (37.5%), and protected against DSSinduced colon inflammation and injury (50% and 66.7%). Pomegranate significantly downregulated phosphorylation of p70S6K, and decreased HIF1 expression. p70S6K is the direct target of miR145. Induction of miR145 by pomegranate juice (2.0 fold) compared to control juice corresponds to the downregulation of p70S6K. Pomegranate significantly attenuated the expresison of proinflammatory cytokines (TNF, Il1 and Il10), COX2 and iNOS. The involvement of miR145/p70S6K/HIF1 was additionally investigated in CCD18Co colonmyofibroblastic cells, where the involvement of this pathway was confirmed using the specific antagomiR of miR145, where the effects of pomegranate polyphenolics were in part reversed by the antagomir.These results suggest that pomegranate intake attenuated DSSinduced colitis by modulating the miR145/p70S6K/HIF1 pathway and inhibiting inflammatory responses, and it may be a potential therapeutic for colitis.Grant Funding Source: Vegetable & Fruit Improvement Center (TAMU)

published proceedings

  • FASEB JOURNAL

author list (cited authors)

  • Kim, H., Banerjee, N., Pfent, C., Talcott, S., & Mertens-Talcott, S.

citation count

  • 0

complete list of authors

  • Kim, Hyemee||Banerjee, Nivedita||Pfent, Catherine||Talcott, Stephen||Mertens‐Talcott, Susanne

publication date

  • April 2013

publisher