Pomegranate Polyphenols Suppress Colorectal Aberrant Crypt Foci (ACF) and Inflammation: Possible role of miR126 in vitro and in vivo Conference Paper uri icon


  • The antitumorigenic efficacy of polyphenols ellagitannins and anthocyanins extracted from pomegranate has been extensively studied in various cancer models. The objective of this study was to investigate the role of posttranscriptional regulation of apoptotic and inflammatory biomarkers by specific microRNAs treated with pomegranate polyphenols (PP).mRNA, microRNA expression were measured by RTPCR, protein by western blotting and multiplex bead assay. In vivo studies were performed with Sprague Dawley rats.PP juice suppressed the number of high multiplicity aberrant crypts foci (HMACF >; 4 ACF) by 42% (P<0.05) and lowered Ki67 antigen. Cell proliferation was also inhibited by PP (525 g/mL) in colon cancer cells HT29. In vitro and vivo results were accompanied by a downregulation of expression including NFB, inducible nitric oxide synthase (iNOS), cyclooxygenase2 (COX2), vascular adhesion molecule1 (VCAM1), insulin growth factor (IGF) and PI3K/Akt/mTOR signaling pathways. miRNA 126 is frequently lost in colon cancer and targets VCAM1 and PI3K. PP increased miR126 expression. In vitro, the involvement of miR126 was confirmed using the antagomir for miR126, where PP reversed the effects of the antagomir.In summary Pg inhibited HT29 cell proliferation, suppressed the number of HMACF formation and colon tumorgenesis where microRNA 126regulated pathways seem to be involved.Grant Funding Source: Vegetable and Fruit Improvement Center, Texas AandM University

published proceedings


author list (cited authors)

  • Banerjee, N., Kim, H., Talcott, S., & Mertens-Talcott, S. U.

citation count

  • 0

complete list of authors

  • Banerjee, Nivedita||Kim, Hyemee||Talcott, Stephen||Mertens-Talcott, Susanne U

publication date

  • April 2013