Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: fabrication, characterization, and enzymatic activity. Academic Article uri icon


  • In this paper we show that hyperbranched polymers can be used as a host matrix for electrostatic entrapment of enzymes. Specifically, amine-functionalized glucose oxidase (GOx+) and horseradish peroxidase, as well as poly(amidoamine) dendrimer-modified horseradish peroxidase, reversibly sorb into polyanionic, hyperbranched poly(sodium acrylate) (PAA-) films that are on the order of a few hundred angstroms thick. The quantity of GOx+ entrapped within the PAA- films depends on the nature of film preparation but is typically on the order of 0.06 unit/cm2. The extent to which entrapped GOx+ retains its activity depends on the film history, but for PAA-/GOx+ composites not exposed to glucose and stored at 4 degrees C, the original activity is retained for up to 68 days and perhaps longer.

published proceedings

  • Anal Chem

author list (cited authors)

  • Franchina, J. G., Lackowski, W. M., Dermody, D. L., Crooks, R. M., Bergbreiter, D. E., Sirkar, K., Russell, R. J., & Pishko, M. V.

citation count

  • 111

complete list of authors

  • Franchina, JG||Lackowski, WM||Dermody, DL||Crooks, RM||Bergbreiter, DE||Sirkar, K||Russell, RJ||Pishko, MV

publication date

  • August 1999