Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography. Academic Article

abstract

• [Structure: see text] The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands, including ligand loading and integrity, was determined by 1H NMR spectroscopy. One of the CsA macroligands was used in a protein affinity experiment to capture known binding proteins of CsA, the cyclophilins, from Jurkat T-cell lysates.

authors

• Bergbreiter, David

published proceedings

• Org Lett

altmetric score

• 3

author list (cited authors)

• Zhou, M., Sivaramakrishnan, A., Ponnamperuma, K., Low, W., Li, C., Liu, J. O., Bergbreiter, D. E., & Romo, D.

citation count

• 11

complete list of authors

• Zhou, Min||Sivaramakrishnan, Ananthapadmanab||Ponnamperuma, Krishan||Low, Woon-Kai||Li, Chunmei||Liu, Jun O||Bergbreiter, David E||Romo, Daniel

publication date

• November 2006

publisher

• American Chemical Society (ACS)  Publisher

published in

• Organic Letters  Journal

keywords

• Chromatography, Affinity
• Cyclophilins
• Cyclosporine
• Dexamethasone
• Glucocorticoids
• Humans
• Immunosuppressive Agents
• Jurkat Cells
• Ligands
• Molecular Structure
• Protein Binding

PubMed Central ID

• 17078689

Digital Object Identifier (DOI)

• 10.1021/ol062045w

start page

• 5247

end page

• 5250

volume

• 8

issue

• 23

URL

• http://dx.doi.org/10.1021/ol062045w