On-demand geometric metering-based multi-reagent mixture generator for robust high-throughput single cell environmental toxin screening Conference Paper uri icon


  • Generating biomolecular mixtures at specified mixing ratios is an essential part in developing high-throughput microfluidic live cell screening platforms. However, producing three or more reagent mixtures require complicated structures and operating procedures when using traditional microfluidic network-based gradient generators. Here, we present an on-demand geometric metering-based mixture generator which facilitates robust, scalable, and accurate multi-reagent mixing in a highthroughput fashion, where mixing ratios are intrinsically determined by the volume ratios of corresponding reagent chambers. A high-throughput live cell array combining this mixture generator with single-cell trapping arrays was implemented for studying combination effects of environmental toxins with single-cell resolution analysis. Copyright (2011) by the Chemical and Biological Microsystems Society.

published proceedings

  • 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

author list (cited authors)

  • Wang, H., Kim, J., Jayaraman, A., & Han, A.

complete list of authors

  • Wang, H||Kim, J||Jayaraman, A||Han, A

publication date

  • December 2011