On-demand geometric metering-based multi-reagent mixture generator for robust high-throughput single cell environmental toxin screening
Conference Paper
Overview
Identity
Additional Document Info
View All
Overview
abstract
Generating biomolecular mixtures at specified mixing ratios is an essential part in developing high-throughput microfluidic live cell screening platforms. However, producing three or more reagent mixtures require complicated structures and operating procedures when using traditional microfluidic network-based gradient generators. Here, we present an on-demand geometric metering-based mixture generator which facilitates robust, scalable, and accurate multi-reagent mixing in a highthroughput fashion, where mixing ratios are intrinsically determined by the volume ratios of corresponding reagent chambers. A high-throughput live cell array combining this mixture generator with single-cell trapping arrays was implemented for studying combination effects of environmental toxins with single-cell resolution analysis. Copyright (2011) by the Chemical and Biological Microsystems Society.