Purification of Monoclonal Antibodies From Plants
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Summary This chapter discusses the advantages and constraints of plants as a platform technology, particularly with respect to downstream processing. It shows generic downstream processing steps for plant tissue expressing monoclonal antibodies (mAbs). These steps include: the disintegration of tissue/cells to release the target mAb; solids separation and clarification; pretreatment of the clarified extract; product capture and purification by affinity chromatography or other biospecific interactions; and product polishing. In mammalian systems, Protein A affinity chromatography has become the industry-wide workhorse for the capture and purification of mAbs, and as would be expected, the use of Protein A has extended to plant systems. The high cost of Protein A resin for the capture of mAbs and Fc fusion proteins has led to the evaluation of alternative process schemes for the purification of mAbs from both mammalian cell cultures and plant tissue extracts.
