Lemon (Citrus lemon L. Burm) as a Source of Unique Bioactive Compounds Conference Paper

abstract

• Citrus contains several bioactive health maintaining compounds that may have potential for prevention of certain diseases. In order to understand the role of these bioactives, isolation and characterization are critical steps of bio-assay derived studies. Lemon seed powder was extracted with hexane and ethyl acetate (EtOAc), using Soxhlet extractor. The EtOAc extract was concentrated by rotary evaporation and dried. The dried residue was extracted with dichloromethane (DCM) and concentrated to obtain limonin crystals. However, β-sitosterol was obtained by silica gel column chromatography. These compounds were detected by thin layer chromatography and purity was confirmed by high-performance liquid chromatography (HPLC). Furthermore, the structures of the isolated compounds were confirmed by atmospheric pressure chemical ionization-mass spectrometry (APCI-MS).

authors

• Patil, Bhimanagouda

published proceedings

• III INTERNATIONAL SYMPOSIUM ON HUMAN HEALTH EFFECTS OF FRUITS AND VEGETABLES - FAVHEALTH 2009

author list (cited authors)

• Kim, J., Jayaprakasha, G. K., & Patil, B. S.

citation count

• 1

complete list of authors

• Kim, J||Jayaprakasha, GK||Patil, BS

publication date

• June 2014

publisher

• International Society for Horticultural Science (ISHS)  Publisher

published in

• Acta Horticulturae  Journal

keywords

• Beta-sitosterol
• Bioactive Compounds
• Lemon
• Limonin

Digital Object Identifier (DOI)

• 10.17660/actahortic.2014.1040.50

International Standard Book Number (ISBN) 13

• 9789462610286

start page

• 377

end page

• 380

volume

• 1040

issue

• 1040

URL

• http://dx.doi.org/10.17660/ActaHortic.2014.1040.50