Circadian and light-induced expression of luciferase in Neurospora crassa Academic Article uri icon

abstract

  • We have constructed a plasmid vector for expressing firefly luciferase in Neurospora crassa under control of the light- and clock-regulated ccg-2 (eas) promoter. The sequence of the luciferase gene in the vector has been modified to reflect the N. crassa codon bias. Both light-induced activity and circadian activity are demonstrated. Expression of luciferase in strains carrying mutant frequency alleles shows appropriate period length alterations. These data demonstrate that luciferase is a sensitive reporter of gene expression in N. crassa. Our results also show that the modified luciferase is expressed in Aspergillus nidulans.

author list (cited authors)

  • Morgan, L. W., Greene, A. V., & Bell-Pedersen, D.

citation count

  • 51

publication date

  • April 2003