Circadian and light-induced expression of luciferase in Neurospora crassa. Academic Article

abstract

• We have constructed a plasmid vector for expressing firefly luciferase in Neurospora crassa under control of the light- and clock-regulated ccg-2 (eas) promoter. The sequence of the luciferase gene in the vector has been modified to reflect the N. crassa codon bias. Both light-induced activity and circadian activity are demonstrated. Expression of luciferase in strains carrying mutant frequency alleles shows appropriate period length alterations. These data demonstrate that luciferase is a sensitive reporter of gene expression in N. crassa. Our results also show that the modified luciferase is expressed in Aspergillus nidulans.

authors

• Bell-Pedersen, Deborah

published proceedings

• Fungal Genet Biol

author list (cited authors)

• Morgan, L. W., Greene, A. V., & Bell-Pedersen, D.

citation count

• 54

complete list of authors

• Morgan, Louis W||Greene, Andrew V||Bell-Pedersen, Deborah

publication date

• January 2003

publisher

• Elsevier  Publisher

published in

• n1087-1845ISSN  Journal

keywords

• Aspergillus Nidulans
• Base Sequence
• Circadian Rhythm
• Fungal Proteins
• Gene Expression Regulation, Fungal
• Genetic Vectors
• Light
• Luciferases
• Molecular Sequence Data
• Neurospora Crassa
• Plasmids

Digital Object Identifier (DOI)

• 10.1016/s1087-1845(02)00562-5

start page

• 327

end page

• 332

volume

• 38

issue

• 3

URL

• http://dx.doi.org/10.1016/s1087-1845(02)00562-5