Serum Amyloid P and a Dendritic Cell-Specific Intercellular Adhesion Molecule-3-Grabbing Nonintegrin Ligand Inhibit High-Fat Diet-Induced Adipose Tissue and Liver Inflammation and Steatosis in Mice. Academic Article uri icon


  • High-fat diet (HFD)-induced inflammation is associated with a variety of health risks. The systemic pentraxin serum amyloid P (SAP) inhibits inflammation. SAP activates the high-affinity IgG receptor Fc receptor I (FcRI; CD64) and the lectin receptor dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN; CD209). Herein, we show that for mice on an HFD, injections of SAP and a synthetic CD209 ligand (1866) reduced HFD-increased adipose and liver tissue inflammation, adipocyte differentiation, and lipid accumulation in adipose tissue. HFD worsened glucose tolerance test results and caused increased adipocyte size; for mice on an HFD, SAP improved glucose tolerance test results and reduced adipocyte size. Mice on an HFD had elevated serum levels of IL-1, IL-23, interferon (IFN)-, IFN-, monocyte chemoattractant protein 1 [MCP-1; chemokine (C-C motif) ligand 2 (CCL2)], and tumor necrosis factor-. SAP reduced serum levels of IL-23, IFN-, MCP-1, and tumor necrosis factor-, whereas 1866 reduced IFN-. Invitro, SAP, but not 1866, treated cells isolated from white fat tissue (stromal vesicular fraction) produced the anti-inflammatory cytokine IL-10. HFD causes steatosis, and both SAP and 1866 reduced it. Conversely, compared with control mice, SAP knockout mice fed on a normal diet had increased white adipocyte cell sizes, increased numbers of inflammatory cells in adipose and liver tissue, and steatosis; and these effects were exacerbated on an HFD. SAPand1866 may inhibit some, but not all, of the effects of a high-fat diet.

published proceedings

  • Am J Pathol

altmetric score

  • 0.5

author list (cited authors)

  • Pilling, D., Cox, N., Thomson, M. A., Karhadkar, T. R., & Gomer, R. H.

citation count

  • 4

complete list of authors

  • Pilling, Darrell||Cox, Nehemiah||Thomson, Megan A||Karhadkar, Tejas R||Gomer, Richard H

publication date

  • January 2019