Evidence for direct actions of melanocortin peptides on bone metabolism. Academic Article

abstract

• Expression of melanocortin-4 receptor (MC4R) mRNA in developing rat limb buds, teeth, and skull bone first indicated a possible role for MC4R in bone metabolism. We therefore investigated whether MC4R mRNA was expressed in the rat osteosarcoma UMR106.06 cell line and in primary rat osteoblast cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), Northern blot analysis, and ribonuclease protection assay (RPA) were used to demonstrate MC4R mRNA expression in UMR106.06 and primary osteoblast cells. MC4R mRNA was found to be localized to the periosteum of mouse bone using in situ hybridization. We also used RT-PCR and rat specific MC2R and MC5R oligonucleotides to amplify the correct size DNA fragments for these melanocortin receptors from rat primary osteoblasts. In conclusion, melanocortin receptor expression in mouse periosteum and rat osteoblasts suggests a direct role for POMC derived peptides in bone development and bone metabolism.

authors

• Wu, Chia Shan

published proceedings

• Peptides

author list (cited authors)

• Dumont, L. M., Wu, C., Tatnell, M. A., Cornish, J., & Mountjoy, K. G.

citation count

• 23

complete list of authors

• Dumont, Laurence M||Wu, C-S Jenny||Tatnell, Michele A||Cornish, Jillian||Mountjoy, Kathleen G

publication date

• October 2005

publisher

• Elsevier  Publisher

published in

• Peptides  Journal

keywords

• Animals
• Blotting, Northern
• Bone And Bones
• Cell Line, Tumor
• Cells, Cultured
• Mice
• Osteoblasts
• Peptides
• Periosteum
• Pro-Opiomelanocortin
• RNA, Messenger
• Rats
• Receptor, Melanocortin, Type 2
• Receptor, Melanocortin, Type 4
• Receptors, Corticotropin
• Receptors, Melanocortin

PubMed Central ID

• 15979763

Digital Object Identifier (DOI)

• 10.1016/j.peptides.2004.12.034

start page

• 1929

end page

• 1935

volume

• 26

issue

• 10

URL

• http://dx.doi.org/10.1016/j.peptides.2004.12.034