BetaSweet carrot extracts have antioxidant activity and in vitro antiproliferative effects against breast cancer cells Academic Article

abstract

• 2019 Elsevier Ltd Fruits and vegetables have long been studied for their potential roles in reducing certain cancer risks. Herein, lyophilized BetaSweet carrots were extracted with hexane, methanol, and water and their chemical constituents were identified by UPLC-QTOF-HR-MS. Methanol extracts exhibited significantly higher radical scavenging activity compared with hexane and water extracts. Hexane extracts showed significantly higher (88.4%) inhibition of breast cancer cells proliferation after 24 h at 100 g/mL, followed by water and methanol extracts, likely due to the presence of polyacetylenes. Cells treated with hexane extracts showed the highest cytochrome-c release, indicating induction of apoptosis, as supported by DNA fragmentation and Bax2 and bcl2 expression and confirmed by staining with propidium iodide and acridine orange. The methanol extract showed the highest antioxidant activity and was purified to obtain cyanidin-3-2-xylosyl-6-(ferulosyl-glucosyl)-galactoside. In conclusion, BetaSweet carrots exhibits multiple health benefits to use as functional foods.

authors

• Patil, Bhimanagouda

published proceedings

• JOURNAL OF FUNCTIONAL FOODS

author list (cited authors)

• Jayaprakasha, G. K., Murthy, K., Pellati, F., & Patil, B. S.

citation count

• 4

complete list of authors

• Jayaprakasha, GK||Murthy, KN Chidambara||Pellati, Federica||Patil, Bhimanagouda S

publication date

• November 2019

publisher

• Elsevier  Publisher

published in

• Journal of Functional Foods  Journal

keywords

• Anthocyanins
• Antiproliferative Activity
• Betasweet Carrots
• MCF-7 Cells
• Polyacetylenes
• Radical Scavenging Activity

Digital Object Identifier (DOI)

• 10.1016/j.jff.2019.103552

start page

• 103552

end page

• 103552

volume

• 62

URL

• http://dx.doi.org/10.1016/j.jff.2019.103552