High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR. Chapter uri icon

abstract

  • An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs against Cryptosporidium parvum in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of C. parvum in vitro.

author list (cited authors)

  • Zhang, H., & Zhu, G.

citation count

  • 10

complete list of authors

  • Zhang, Haili||Zhu, Guan

Book Title

  • Methods in Molecular Biology

publication date

  • August 2020