In Vitro Differentiation of T Cells from Murine Pluripotent Stem Cells. Academic Article

abstract

• In recent years cancer immunotherapy, especially the cell-based immunotherapy, has reached several milestones and achieved a lot of cancer remission in the clinics. Obtaining a more potent and effective cytotoxic T lymphocytes (CTLs) for cancer immunotherapy is always the ultimate goal for the researchers. However, the difficulty in harvesting a large number of tumor antigen-specific CTLs from the tumor patient is still a major obstacle we need to overcome. In our previous studies, it is shown that pluripotent stem cell-derived CTL-especially the genetically engineered antigen-specific CTLs-may serve as a good source of unlimited number of highly reactive and antigen-specific CTLs. Here we present a two-step method for the generation of antigen-specific T lymphocytes from iPS cells by in vitro priming and in vivo maturation.

authors

• Song, Jianxun

published proceedings

• Methods Mol Biol

author list (cited authors)

• Chen, X., Lei, F., Wang, L., Xiong, X., & Song, J.

citation count

• 1

publication date

• January 2019

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Cell Culture Techniques
• Coculture Techniques
• Culture Media
• Differentiation
• Disease Models, Animal
• Female
• Flow Cytometry
• Genetic Vectors
• Homeodomain Proteins
• Induced Pluripotent Stem Cells
• Melanoma, Experimental
• Mice
• Mice, Knockout
• Notch Signaling
• Protein Engineering
• Receptors, Antigen, T-Cell
• Retroviridae
• Stem Cell Biology
• T-Lymphocytes, Cytotoxic
• Transduction, Genetic

PubMed Central ID

• 31396937

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-9728-2_14

start page

• 131

end page

• 141

volume

• 2048

URL

• http://dx.doi.org/10.1007/978-1-4939-9728-2_14